A Simultaneous Coupling Azo Dye Technique Suitable for Whole Mounts
نویسنده
چکیده
A method based on the familiar coupling azo dye technique has been devised for the study of enzyme distribution in thick specimens. Its application to the study of esterase distribution in whole tadpoles of Xenopus laevis is described in detail. It can also be used to show the distribution of alkaline phosphatase, both in Xenopus tadpoles and in limbs dissected from chick embryos. It has proved useful in embryological studies both for teaching and for research.
منابع مشابه
Bi(NO3)3.5H2O: An efficient acidic reagent for synthesis of azo dyes at room temperature
An one-pot method has been developed for the synthesis of azo dyes via sequential diazotization–diazo coupling of aromatic amines with coupling agents at room temperature in the presence of Bi(NO3)3. 5H2O. Short reaction times, high yields, a clean process, simple methodology, easy work-up and green conditions are advantages of this protocol.
متن کاملBi(NO3)3.5H2O: An efficient acidic reagent for synthesis of azo dyes at room temperature
An one-pot method has been developed for the synthesis of azo dyes via sequential diazotization–diazo coupling of aromatic amines with coupling agents at room temperature in the presence of Bi(NO3)3. 5H2O. Short reaction times, high yields, a clean process, simple methodology, easy work-up and green conditions are advantages of this protocol.
متن کاملNovel Naphthalimide Based Azo Disperse Dyes for Dyeing of Polyester Fabrics
A series of monoazo disperse dyes based on naphthalimide was synthesized using N-alkylcarboxylic acid-1,8-naphthalimide as diazo component and N,N-diethylaniline derivatives as a coupling components. 4-amino-N-ethanoic (propanoic) acid-1,8-naphthalimide was synthesized from 4-nitro-1,8-naphthalic anhydride by imidation and subsequent reduction reactions. These compounds were diazotized and furt...
متن کاملPhotocatalytic Degradation of Azo Dye Acid Red 14 from Aqueous Solutions Using MWCNTs Nanocatalyst
Background & Aims of the Study: Azo Dyes are the most hazardous materials in different industries. Dyes and pigments used in industries for applications such as textiles, leathers, papers, foodstuffs, additives, etc. Application amounts of azo dyes in industries which can cause severe health problems in human and environmental pollutant problems. So, color wastewaters decomposition plan are...
متن کاملSpectrophotometric Determination of Ascorbic acid in Aqueous Solutions and in Pharmaceuticals formulations
A new sensitive colorimetric method for determination of ascorbic acid tablet in aqueous solution. The method is based on the formation of colored azo dye by diazotization of 2, 4dichloroaniline followed by azo-coupling reaction between the resulting product and ascorbic acid. The optimum conditions that gave maximum absorption of azo dye at535nm, with best concentration of reactant and order o...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2006